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flow cytometry  (Miltenyi Biotec)


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    Miltenyi Biotec flow cytometry
    Flow Cytometry, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1932 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry/product/Miltenyi Biotec
    Average 98 stars, based on 1932 article reviews
    flow cytometry - by Bioz Stars, 2026-05
    98/100 stars

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    Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Journal: STAR Protocols

    Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

    doi: 10.1016/j.xpro.2026.104471

    Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

    Techniques: Flow Cytometry, Isolation, Staining

    Synthesis, characterization, and exosomes release profile of Exos-AlgMA hydrogels. (A) Schematic diagram of the synthesis of AlgMA. (B) The 1 H NMR spectra of Alg and AlgMA. (C) Compressive stress-strain curves and (D) corresponding compressive moduli of AlgMA and Exos-AlgMA hydrogel. (n = 3; ns: no significance). (E) SEM micrograph of the porous structure of the AlgMA hydrogel. Scale bar: 50 μm. (F) TEM image of BMSC-derived exosomes. Scale bar: 50 nm. (G) Particle size distribution of exosomes measured by DLS. (H) Cumulative release profiles of exosomes from the Exos-AlgMA hydrogel over 10 days. (I, J) Bead-assisted flow cytometry analysis of exosomal markers (CD9 and CD63). Percentages indicate CD9 + or CD63 + events within the bead gate, with unstained/blank controls used to define positive thresholds.

    Journal: Materials Today Bio

    Article Title: The mechanistic study of injectable hydrogel loaded with BMSC-exosomes in regulating the TGF-β/MMP axis to inhibit experimental myopia model

    doi: 10.1016/j.mtbio.2026.103051

    Figure Lengend Snippet: Synthesis, characterization, and exosomes release profile of Exos-AlgMA hydrogels. (A) Schematic diagram of the synthesis of AlgMA. (B) The 1 H NMR spectra of Alg and AlgMA. (C) Compressive stress-strain curves and (D) corresponding compressive moduli of AlgMA and Exos-AlgMA hydrogel. (n = 3; ns: no significance). (E) SEM micrograph of the porous structure of the AlgMA hydrogel. Scale bar: 50 μm. (F) TEM image of BMSC-derived exosomes. Scale bar: 50 nm. (G) Particle size distribution of exosomes measured by DLS. (H) Cumulative release profiles of exosomes from the Exos-AlgMA hydrogel over 10 days. (I, J) Bead-assisted flow cytometry analysis of exosomal markers (CD9 and CD63). Percentages indicate CD9 + or CD63 + events within the bead gate, with unstained/blank controls used to define positive thresholds.

    Article Snippet: Exosome surface markers were analyzed using a bead-assisted flow cytometry method.

    Techniques: Derivative Assay, Flow Cytometry